Table 18. Recipe of crush soak buffer .

Reagents	Initial Concentration	Final Concentration	Dilution Fold	Final Volume (10 mL)
NaCl	5,000 mM	500 mM	10	1,000 μL
EDTA	500 mM	1 mM	500	20 μL
SDS	10%	0.50%	20	500 μL
Nuclease-free water	/	/	/	8,480 μL