Fig. 5.

The apoptotic effect of C4 on HT-29 colon cancer cells was quantified by Annexin V-PI staining assay. (A) Flow cytometry charts of HT-29 cells representing untreated control, treated with 5 µM C4 and treated with 10 µM C4, respectively. Graphs show (B) percent apoptosis and (C) percent total cell death of HT-29 cells following treatment with 5 µM and 10 µM C4 concentrations. Results are expressed as mean±SD of three independent experiments. **p < 0.01 and ***p < 0.001 for HT-29 cells versus untreated control were considered significant.