Figure 4.

SIRT6 regulates the malignant phenotype of pancreatic cancer cells by activating the NF-κB pathway. (A) Nuclear NF-κB p65 and cytoplasmic IκBα protein levels were detected by western blot analysis. (B) Nuclear NF-κB p65 expression was evaluated by immunofluorescence. (C) PANC-1 cells were treated with vector, SIRT6, and SIRT6 + RANKL, and then nuclear NF-κB p65 and cytoplasmic IκBα protein levels were assessed by western blotting. (D) The viability of PANC-1 cells was evaluated by Cell Counting Kit-8 assay. (E) ROS level was detected by DCFH-DA method. (F) The expression levels of GPX4, SLC7A11, HK2 and LDHA were determined by western blotting. ^**P<0.01 vs. vector group and ^&P<0.05 vs. SIRT6 group. SIRT6, sirtuin6; ROS, reactive oxygen species; GPX4, glutathione peroxidase 4; HK, hexokinase; LDHA, lactate dehydrogenase A.