Figure 5.

Upregulation of SIRT6 inhibits growth of pancreatic cancer xenografts in vivo. (A) Different groups of PANC-1 cells (vector and SIRT6-overexpressing) were inoculated subcutaneously (200 ml) in nude mice at a density of 1x10⁶/ml and the xenografted tumor size was measured every 7 days. After 28 days, the tumor was stripped from euthanized mice, and the tumor growth curve was drawn. (B and C) The body weight and xenografted tumor mass of nude mice were calculated. (D) The expression levels of GPX4, SLC7A11, HK2 and LDHA were assessed by immunohistochemistry. (E) Nuclear NF-κB p65 and cytoplasmic IkBα protein levels were detected by western blot analysis. ^**P<0.01 vs. vector group. SIRT6, sirtuin6; GPX4, glutathione peroxidase 4; HK, hexokinase; LDHA, lactate dehydrogenase A.