Table 3.
Transcriptome data for complement resistance genes showing genes with differential expression in human sera compared to nutrient rich media in the complement resistant NPRC-AB20 strain and the intermediate sensitivity AB1615-09 and AB1 strains.
| Serum resistance genes | Putative gene function | Log2 Fold change in expression levels in NHS compared to broth | |||||
|---|---|---|---|---|---|---|---|
| AB1615-09 | AB1 | NPRC-AB20 | |||||
| Fold change | P-value1 | Fold change | P-value1 | Fold change | P-value1 | ||
| Upregulated genes in the NPRC-AB20 strain | |||||||
| ABUW_2168 | hypothetical protein | 2.52 | 2.79E-12 | 2.36 | 5.70E-09 | 2.87 | 2.58E-05 |
| ABUW_2637 | Hypothetical protein | 0.86 | 3.08E-11 | 0.86 | 0.000400 | 1.23 | 1.12E-10 |
| ABUW_0385 | toluene tolerance efflux transporter | 1.07 | 0.00231029 | 1.64 | 3.05E-09 | 0.89 | 0.0001013 |
| ABUW_0729 | uppP | 0.09 | 0.64808354 | 0.11 | 0.6985674 | 0.51 | 0.0121933 |
| Downregulated genes in the NPRC-AB20 strain | |||||||
| ABUW_2456 | Putative Hydroxymethylglutaryl-CoA lyase | -2.10 | 2.86E-52 | -2.14 | 1.50E-14 | -3.47 | 6.80E-30 |
| ABUW_3639 | response regulator | 0.22 | 0.48382633 | 0.35 | 0.304992 | -0.85 | 0.000370 |
| ABUW_1759 | Extracellular serine proteinase | -0.93 | 6.19E-12 | -0.93 | 8.58E-10 | -0.85 | 1.71E-06 |
| ABUW_3822 | Serine acetyltransferase | 0.27 | 0.3983097 | 0.64 | 5.84E-05 | -0.66 | 0.000789 |
1 P-value for expression in serum versus broth.
2Genes in bold are show differential relative expression levels between strain NPRC-AB20 and AB1615-09 and AB1.