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Degradation of PLA film by the purified PLA depolymerase. PLA films (thickness, 5 μm) were treated with the purified PLA depolymerase (140 μg/ml) in a dialysis tube at 37°C for 48 h or left untreated, as described in Materials and Methods. Scanning electron micrographs of an untreated film at a ×1,800 magnification (A) and an enzyme-treated film at magnifications of ×1,800 (B) and ×180 (C) are shown.
