Fig. 6.

HHLA2 overexpression reverses the inhibition of tumor growth mediated by METTL3 depletion. The subcutaneous transplantation tumor model was used to study whether HHLA2 overexpression could reverse the inhibition of tumor growth mediated by METTL3 depletion. In 786-O cells, LV-NC, LV-METTL3-shRNA, LV-NC + LV-HHLA2^OE, and LV-METTL3-shRNA + LV-HHLA2^OE subgroups (1 × 10⁷ in 150 µL PBS) were used to establish the tumor model in nude mice (4 ~ 5 weeks old). A and B Depletion of METTL3 could significantly decrease the tumor growth, and overexpression of HHLA2 could significantly increase the tumor growth. Overexpression of HHLA2 in the METTL3-depleted model could significantly reverse and promote the tumor growth. C The tumor weights of different subgroups were also evaluated. Consistent with the tumor growth curves, depletion of METTL3 could significantly decrease the tumor weights, while it could be reversed by overexpression of HHLA2 in 786-O cells. Un-paired t test and Two-way ANOVA analyses were used respectively. ^**P < 0.01, ^****P < 0.0001