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. Author manuscript; available in PMC: 2022 Jul 6.
Published in final edited form as: Sci Transl Med. 2022 May 25;14(646):eabn1252. doi: 10.1126/scitranslmed.abn1252

Fig 5. Top multivalent minibinder candidates are resistant to viral escape.

Fig 5.

(A) Plaque assays were performed to isolate VSV-SARS-CoV-2 S chimera virus escape mutants against a control neutralizing antibody (2B04) and the FUS231-P12 and TRI2–2 multivalent minibinders. For each inhibitor tested, Vero CCL-81 cells were incubated with VSV-SARS-CoV-2 S chimera virus for one hour, followed by addition of the inhibitor protein at a fully neutralizing concentration and further incubation to allow for replication and spread of resistant viruses. Thirty-six independent selections were carried out for each minibinder compound in a single experiment; representative examples are shown in the images. Small plaques are indicative of inhibited viral spreading and large plaques, highlighted by black arrows, are indicative of viral escape mutants spreading. (B) A summary of the results of the viral escape screen are shown. NAb, neutralizing antibody.