TABLE 6.

Plasma HDL subfractions of young and older adults at baseline and older adults before and after 6 mo of placebo or n–3-PUFA supplementation¹

								Old n–3 PUFA vs. old placebo
			Old n–3 PUFA (n = 22)		Old placebo (n = 22)		Young vs. old	Adjusted mean
	Young (n = 30)	Old (n = 54)	Baseline	Follow-up	Baseline	Follow-up	P value	difference	95% CI	P value
Triglycerides, HDL-1, mg/dL	2.43 ± 1.59	3.30 ± 1.30	2.90 ± 0.98	3.51 ± 1.31	3.65 ± 1.55	3.67 ± 1.69	0.009	- 0.344	−1.14, 0.45	0.387
Triglycerides, HDL-2, mg/dL	1.40 ± 0.58	1.61 ± 0.50	1.48 ± 0.38	1.74 ± 0.47	1.71 ± 0.61	1.74 ± 0.56	0.084	−0.103	−0.397, 0.192	0.485
Triglycerides, HDL-3, mg/dL	1.84 ± 0.58	1.93 ± 0.55	1.85 ± 0.52	1.73 ± 0.42	2.01 ± 0.61	2.03 ± 0.52	0.468	0.23	−0.019, 0.471	0.069
Triglycerides, HDL-4, mg/dL	3.07 ± 0.80	3.20 ± 0.97	3.25 ± 0.98	2.30 ± 0.84	3.23 ± 0.98	3.11 ± 0.80	0.542	0.83	0.507, 1.15	<0.001
Cholesterol, HDL-1, mg/dL	15.1 ± 7.28	18.3 ± 8.30	16.6 ± 7.64	22.0 ± 8.86	20.0 ± 8.91	20.5 ± 9.29	0.086	−4.45	−7.87, −1.02	0.012
Cholesterol, HDL-2, mg/dL	7.63 ± 2.50	8.30 ± 2.83	7.82 ± 2.63	10.0 ± 3.24	8.78 ± 3.19	9.35 ± 2.95	0.281	−1.463	−2.68, −0.246	0.020
Cholesterol, HDL-3, mg/dL	10.1 ± 2.08	10.2 ± 2.51	10.0 ± 2.25	10.2 ± 2.16	10.4 ± 3.0	11.1 ± 2.53	0.898	0.68	−0.363, 1.72	0.196
Cholesterol, HDL-4, mg/dL	19.1 ± 3.41	18.9 ± 2.82	19.9 ± 2.95	15.3 ± 2.35	18.4 ± 2.53	18.8 ± 2.81	0.793	4.414	3.12, 5.71	<0.001
Free cholesterol, HDL-1, mg/dL	3.40 ± 1.79	3.86 ± 1.92	3.40 ± 1.83	5.18 ± 2.20	4.20 ± 2.02	4.34 ± 2.16	0.281	−1.60	−2.37, −0.824	<0.001
Free cholesterol, HDL-2, mg/dL	1.69 ± 0.64	1.63 ± 0.64	1.48 ± 0.66	1.92 ± 0.70	1.73 ± 0.66	1.82 ± 0.66	0.751	−0.31	−0.583, −0.029	0.031
Free cholesterol, HDL-3, mg/dL	1.81 ± 0.53	1.76 ± 0.62	1.68 ± 0.61	1.57 ± 0.56	1.85 ± 0.70	1.87 ± 0.66	0.724	0.19	−0.067, 0.447	0.144
Free cholesterol, HDL-4, mg/dL	3.35 ± 0.81	3.27 ± 0.78	3.38 ± 0.80	2.60 ± 0.66	3.22 ± 0.80	3.19 ± 0.75	0.659	0.69	0.4, 0.98	<0.001
Phospholipids, HDL-1, mg/dL	18.6 ± 9.80	22.1 ± 10.0	19.6 ± 9.29	25.1 ± 10.7	24.2 ± 10.6	25.3 ± 11.2	0.128	−3.95	−8.02, 0.13	0.057
Phospholipids, HDL-2, mg/dL	12.4 ± 4.12	13.3 ± 4.10	12.5 ± 3.85	14.5 ± 4.50	14.1 ± 4.60	15.1 ± 4.22	0.348	−0.721	−2.48, 1.04	0.414
Phospholipids, HDL-3, mg/dL	16.5 ± 3.54	16.5 ± 3.76	16.1 ± 3.52	14.7 ± 3.14	17.0 ± 4.31	18.1 ± 3.99	0.974	2.79	1.25, 4.32	0.001
Phospholipids, HDL-4, mg/dL	27.4 ± 3.73	27.3 ± 3.52	28.1 ± 3.80	20.8 ± 2.77	26.9 ± 3.10	27.7 ± 3.89	0.857	7.56	5.86, 9.26	<0.001
ApoA-1, HDL-1, mg/dL	20.2 ± 14.2	25.4 ± 13.9	21.9 ± 12.8	30.6 ± 15.8	28.2 ± 14.6	30.0 ± 16.2	0.111	−6.801	−12.3, −1.3	0.017
ApoA-1, HDL-2, mg/dL	17.1 ± 4.75	18.3 ± 4.54	17.5 ± 4.22	17.5 ± 4.13	19.2 ± 5.07	20.4 ± 4.64	0.253	1.69	−0.095, 3.47	0.063
ApoA-1, HDL-3, mg/dL	26.7 ± 5.13	27.7 ± 5.33	27.2 ± 5.02	25.6 ± 4.50	28.3 ± 6.10	30.1 ± 5.86	0.426	3.72	1.55, 5.89	0.001
ApoA-1, HDL-4, mg/dL	70.6 ± 9.97	71.7 ± 9.37	74.6 ± 9.66	59.6 ± 7.50	69.9 ± 8.82	71.4 ± 9.19	0.633	14.48	10.3, 18.6	<0.001
ApoA-2, HDL-1, mg/dL	1.97 ± 1.44	2.16 ± 1.31	1.74 ± 1.17	2.35 ± 1.41	2.53 ± 1.31	2.63 ± 1.45	0.535	−0.46	−1, 0.083	0.095
ApoA-2, HDL-2, mg/dL	3.05 ± 1.06	2.86 ± 0.97	2.59 ± 0.84	2.73 ± 0.94	3.11 ± 1.13	3.19 ± 1.17	0.394	0.113	−0.414, 0.640	0.667
ApoA-2, HDL-3, mg/dL	6.11 ± 1.32	5.49 ± 1.35	5.33 ± 1.17	5.26 ± 1.17	5.66 ± 1.69	5.83 ± 1.74	0.043	0.38	−0.375, 1.13	0.315
ApoA-2, HDL-4, mg/dL	18.1 ± 3.63	16.7 ± 3.10	17.7 ± 3.24	12.3 ± 2.91	16.0 ± 3.05	16.0 ± 3.14	0.060	4.72	3.20, 6.23	<0.001

¹Values are mean ± SD unless otherwise indicated.