Figure 1.

Generation of the AMM/S stable cell line. (A) Schematic of the donor vector carrying SDF‐1 and donor plasmid DNA targeting the AAVS1 locus in the host genome. Primers F and R indicate primer locations for junction detection. Abbreviations: HA‐L, left homology arm; HA‐R, right homology arm; PGK, phosphoglycerate kinase promoter; EF1α, elongation factor‐1 alpha promoter; Puro, puromycin. (B) GFP expression in AMM/S. Transfected cells were selected by incubating with puromycin and then purified by fluorescence‐activated cell sorting. (C) Confirmation of correct knock‐in of donor plasmid into the AAVS1 locus by junction PCR. (D) SDF‐1 expression levels in the AMM/S cell line assessed by RT‐qPCR. **p < 0.01, n = 4 in each group