FIGURE 6.
UBE2C modulates invasion/migration through HIF‐1α signalling. (A) Differentially regulated HIF‐1α targets genes following UBE2C knockdown in PC3 cells. The colour (green) indicates the degree of downregulation following UBE2C knockdown. (B) Relative expression of HIF‐1α in SAS and CAL27 cells after UBE2C knockdown. Top, western blot analysis; Bottom, RT‐qPCR. (C) CAL27/shUBE2C cells were treated with CoCl2 (as HIF‐1α inducer) for 48 h and analysed by RT‐qPCR. (D) Treatment with or without CoCl2 and migration/invasion potential of CAL27/shUBE2C cells. Data are presented as the mean ± SD. *p < 0.05; **p < 0.01