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. 2022 Jun 1;26(13):3702–3715. doi: 10.1111/jcmm.17398

FIGURE 4.

FIGURE 4

Silencing of SIRT3 partially abolishes the protective effects of MAT on mitochondrial dysfunction and oxidative injury induced by cisplatin in HK2 cells. Cells were transfected with control siRNA (si‐NC) or SIRT3 siRNA (si‐SIRT3) for 6 h and treated with cisplatin in the presence or absence of MAT for 48 h. (A) The expression of SIRT3 was determined by Western blotting. (B) Acetylation of OPA1 in HK2 cells. (C) Representative immunofluorescence of mitochondrial morphology in HK2 cells. The mtROS level (D) and the ATP content (E) in HK2 cells. (F) The MDA content and CAT activity in cells were measured by chemical kit. The data are presented as the means ± SEM (n = 3). **p < 0.01 compared with CON group; # p < 0.05 and ## p < 0.01 compared with Cis group; & p < 0.05 and && p < 0.01 compared with Cis + MAT group