FIGURE 6.

Matrine treatment alleviates nephrotoxicity and apoptosis induced by cisplatin in mice. (A) The body weight, (B) the BUN level and (C) the serum CREA at 4 days after cisplatin injection. (D) Morphological changes of kidney tissues from different group were measured by haematoxylin and eosin and PAS staining. Arrows indicate acute tubular necrosis; pound signs indicate vacuolization. (E and F) The apoptosis in kidney tissues from mice was measured by TdT‐mediated dUTP nick‐end labelling (TUNEL) assay, and the TUNEL‐positive apoptotic cells were quantified by Image‐J software. (G) Western blotting and quantification analysis of apoptosis‐related BAX and Bcl‐2 protein levels. All data are quantified from 6 mice/group and shown as the means ± SEM. **p < 0.01 versus normal control (NC); ^## p < 0.01 versus Cis