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. 2022 Jul 6;17(7):e0271029. doi: 10.1371/journal.pone.0271029

Fig 3. Time-course mRNA abundance of ostf1 in gills of milkfish transferred from FW to SW and SW to FW for 24 h.

Fig 3

(A, B) ostf1a and (C, D) ostf1b was detected by real-time PCR. The expression has been normalized according to the expression of β-actin. The time-points of sampling are 0, 3, 6, 12 and 24 h, respectively. Values were shown in means ± S.E.M. (n = 6). The asterisks indicated significant differences between each time-points and 0 h (p < 0.05, analyzed by Kruskal–Wallis test with Dunn’s multiple comparisons test).