Table 1.
Number of metabolite features meeting QC criteria, number and proportion reported as having overall significant variation between breeds by one-way ANOVA (No. features row) and number of metabolite features reported as being statistically significant in Tukey post hoc analyses (pairwise breed comparisons, p < 0.05).
| HILIC + | HILIC— | LIPIDS + | LIPIDS— | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| DBS (20) | DBS (40) | Plasma | DBS (20) | DBS (40) | Plasma a | DBS (20) | DBS (40) | Plasma | DBS (20) | DBS (40) | Plasma | |
| Total no. features | 3,335 | 3,813 | 3,738 | 973 | 1,277 | 1,276 | 5,013 | 6,180 | 5,817 | 3,590 | 4,018 | 4,166 |
| No. of significant | 424 | 524 | 464 | 78 | 98 | 38 | 407 | 975 | 686 | 580 | 946 | 746 |
| Percentage | 12.71% | 13.74% | 12.41% | 8.02% | 7.67% | 2.98% | 8.12% | 15.78% | 11.79% | 16.16% | 23.54% | 17.91% |
| Norfolk—Labrador | 361 | 423 | 390 | 66 | 83 | 27 | 353 | 781 | 530 | 473 | 727 | 595 |
| Norfolk—PBGV | 244 | 288 | 327 | 46 | 72 | 28 | 344 | 848 | 520 | 467 | 773 | 502 |
| Norfolk—Beagle | 280 | 347 | 321 | 61 | 78 | 27 | 337 | 814 | 476 | 402 | 718 | 443 |
| Labrador—PBGV | 177 | 225 | 154 | 30 | 33 | 14 | 94 | 269 | 207 | 172 | 280 | 338 |
| Labrador—Beagle | 167 | 213 | 171 | 23 | 27 | 18 | 50 | 114 | 216 | 175 | 265 | 339 |
| PBGV—Beagle | 78 | 82 | 63 | 16 | 13 | 6 | 70 | 143 | 117 | 129 | 161 | 110 |
a
Number of representative samples reduced by 5, 4, 3, 2, and 2 for Norfolk Terriers, Petit Basset Griffon Vendeen (PBGV), DSH, Beagle, and Labrador retriever for HILIC negative plasma samples. The critical p-value for one-way-ANOVA was p > 0.005 after correction for multiple testing.