Figure 6.
Antioxidant and bioactive effects of SBE fractionated by HPLC. (a) Peroxyl radical scavenging capacity (PSC) of reconstituted HPLC eluate pools I-V. Equal volumes of reconstituted pools were used in the assay. Results indicate the mean + s.d. of two experiments. *indicates value is different than methanol control at p < 0.05. (b) Effect of reconstituted HPLC eluate pools I-V on glutathione levels in HepG2 cells. Cells were treated with reconstituted HPLC fractions at a dilution which was equivalent to that used to give 100 μM of the original SBE, an equal volume of methanol (cv), or left untreated (ctr) for 12 hours. Results indicate the mean ± s.d. of five experiments. *indicates value different from cv at p < 0.05.