Table 3.

Comparison of HPLC retention times for reference compounds versus SBE pools I-V using the same preparative scale HPLC protocol in each case. Eluting compounds were detected by absorbance at 280 nm.

Reference cpd	Observed ret. time (min)	SBE pool	Collection time (min)
		Pool I	2.50–12.50
Aesculin	21.84	Pool II	12.50–22.50
Aesculetin	30.85	Pool III	22.40–42.50
Isoscopoletin	34.15
Scopoletin	34.79
Umbelliferone	35.95
Quercetagetin	40.31
Apigenin-7-O-glucoside	40.76
Quercetin	49.28	Pool IV	42.50–62.50
Naringenin	51.13
Luteolin	51.57
Eupafolin	52.2
Kaempferol	55.41
		Pool V	62.50–67.5