Fig. 3 |. T-bet deficiency significantly diminishes T_EFF subset formation and function.

a,b,d, Representative flow plots and summary data depicting the percentage of virus-specific CD8⁺ T cells as well as proportions of the three CD8⁺ subsets in WT and Tbx21^−/− BMC mice on days 21–30 p.i with LCMV Cl13. Q, quartile. c, Summary data showing the proportion of CD8⁺ T cells degranulating (CD107a⁺) and producing IFN-γ upon ex vivo stimulation with the GP_33–41 peptide. e, Summary data showing the relative expression of inhibitory molecules and TFs in the three virus-specific CD8⁺ T cell subsets. gMFI, geometric mean fluorescence intensity. f, Summary data showing viral titers in the sera of experimental mice on day 21 p.i. b, Blood was collected from three WT and four Tbx21^−/− BMC mice. Spleens were collected from seven WT and eight Tbx21^−/− BMC mice. c, Data were collected from six WT and seven Tbx21^−/− BMC mice. d, Data were collected from seven WT and eight Tbx21^−/− BMC mice. e, Data were collected from four WT and four Tbx21^−/− BMC mice. f, Data were collected from eight WT and eight Tbx21^−/− BMC mice. FFU, focus-forming units. b–f, Data were pooled from two independent experiments. Data are expressed as mean ± s.e.m. NS, not significant; *P < 0.05, **P < 0.01, ***P < 0.001. b,d,e, Unpaired t-test with the Benjamini and Hochberg method or the Holm–Šídák method. c,f, Two-tailed unpaired t-test.