Fig. 6 |. BATF is required for T_PRo cell differentiation into T_EFF cells.

a–f, Representative flow plots and summary data showing the percentage of virus-specific CD8⁺ T cells as well as the proportion of three antigen-specific CD8⁺ T cell subsets in WT and BATF-HET mice on day 8 or day 21 p.i. with LCMV Cl13. g, Summary data showing the proportion of CD8⁺ T cells degranulating (CD107a⁺) and producing IFN-γ upon ex vivo stimulation with the GP_33–41 peptide. h–j, Summary data showing the relative expression of GzmB, T-bet and PD-1 in three virus-specific CD8⁺ T cell subsets. These subsets were all gated on CD8⁺CD44⁺GFP⁺ cells first, which represent polyclonal LCMV-specific CD8⁺ T cells. c,e, Data were collected from four WT and seven BATF-HET mice. d,f, Data were collected from six WT and four BATF-HET mice. g–j, Data were collected from four WT and four BATF-HET mice. c–j, Data were pooled from two independent experiments. Data are expressed as mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001. c,d, Two-tailed unpaired t-test. e–j, Unpaired t-test with the Benjamini and Hochberg method or the Holm–Šídák method.