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. Author manuscript; available in PMC: 2022 Jul 30.
Published in final edited form as: Methods Enzymol. 2021 Jul 30;658:277–310. doi: 10.1016/bs.mie.2021.06.026
In vitro pseudouridylation reaction:
90 μL 5x Pseudouridylation buffer
10 μL 100mM DTT
2.4 μL recombinant PUS (130 μM) or (~100μg of nuclear extract)
46.5 μL of folded RNA
Bring volume to 500 μL with water.