TABLE 3.
Further quantification of bacteria by FISH and rRNA slot blot hybridization in Smeerenburgfjorden sediments (core A/core B)
| Depth (cm) | β-Proteobacteria, probe BET42a
|
γ-Proteobacteria (probe GAM42a)
|
Clone sequences and symbionts affiliated with the γ-Proteobacteria (probe GAM660)
|
Planctomycetales (probe PLA886)
|
Gram-positive bacteria (probe GP 1199)
|
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| % Prokaryotic RNA | ng of RNA ml−1 | % Total DAPI cell counts | % Prokaryotic RNA | ng of RNA ml−1 | % Total DAPI cell counts | % Prokaryotic RNA | ng of RNA ml−1 | % Total DAPI cell counts | % Universal RNA | ng of RNA ml−1 | % Prokaryotic RNA | ng of RNA ml−1 | |
| 0.25 | 1.6/1.7 | 161/155 | 12.1/9.0 | 17.6/19.4 | 1,739/1,744 | 2.9/2.9 | 11.7/6.9 | 1,153/621 | 2.2/1.9 | 30.7/17.0 | 4,815/3,131 | 0/0.2 | 0/17 |
| 0.75 | 1.8/1.7 | 180/229 | 11.6/9.2 | 20.7/19.4 | 2,075/2,813 | 1.8/2.7 | 9.5/10.5 | 950/1,449 | 3.0/2.8 | 23.5/16.1 | 3,464/3,684 | 0/0.4 | 0/52 |
| 1.25 | 1.8/1.5 | 132/159 | 8.9/9.5 | 22.2/17.8 | 1,626/1,888 | 2.5/2.8 | 11.6/10.8 | 851/1,142 | 3.6/3.5 | 53.6/19.7 | 7,468/4,037 | 0/0.5 | 0/49 |
| 1.75 | 1.4/1.6 | 99/134 | 8.0/6.9 | 20.2/16.8 | 1,388/1,402 | 0.8/2.0 | 17.0/8.2 | 1,170/682 | 2.8/2.7 | 26.0/20.6 | 2,975/2,683 | 0.1/1.4 | 4/116 |
| 2.25 | 1.3/1.6 | 96/158 | 7.3/6.2 | 17.5/16.2 | 1,254/1,558 | 1.6/3.1 | 10.8/11.4 | 772/1,094 | 3.7/3.8 | 19.1/17.2 | 2,154/3,204 | 0.5/1.3 | 35/129 |
| 2.75 | 1.1/1.2 | 58/72 | 5.5/6.1 | 14.5/13.3 | 733/780 | 1.2/2.1 | 18.1/6.1 | 910/359 | 2.8/3.9 | 15.8/13.1 | 1,229/1,091 | 0.7/2.2 | 36/129 |
| 3.25 | 1.3/1.2 | 60/75 | 4.7/4.7 | 14.5/12.5 | 686/804 | 2.6/2.7 | 18.4/6.7 | 871/431 | 3.5/3.3 | 19.1/14.1 | 1,578/1,287 | 0.3/2.6 | 16/167 |
| 3.75 | 1.3/1.1 | 70/77 | 8.7/6.0 | 14.7/12.6 | 764/841 | 2.6/2.8 | 20.1/9.0 | 1,050/599 | 3.1/3.6 | 19.3/13.5 | 1,524/1,257 | 0.2/2.1 | 13/137 |
| 4.25 | 1.1/1.1 | 40/57 | 4.2/4.2 | 13.7/11.8 | 495/596 | 2.2/2.5 | 17.6/7.0 | 635/353 | 1.7/1.9 | 16.2/15.2 | 964/984 | 0/1.2 | 0/63 |
| 4.75 | 1.3/1.1 | 47/45 | 5.1/3.6 | 13.4/11.5 | 483/509 | 2.2/2.7 | 20.4/9.5 | 734/423 | 1.8/1.8 | 26.5/14.0 | 1,475/831 | 0/0.7 | 0/31 |
| 5.5 | 1.2/1.1 | 31/45 | 4.4/5.0 | 11.9/12.0 | 311/486 | 2.8/2.4 | 14.2/14.0 | 3,730/565 | 2.9/1.5 | 21.8/16.0 | 720/793 | 0/0.3 | 0/13 |
| 6.5 | 1.2/1.2 | 36/56 | 5.8/4.0 | 11.2/11.6 | 336/520 | 1.8/2.1 | 16.6/14.2 | 496/638 | 2.5/3.1 | 27.8/14.3 | 931/701 | 0/0.1 | 0/6 |
| 7.5 | 1.2/1.1 | 31/38 | 6.4/3.0 | 9.3/11.8 | 246/415 | 2.4/1.3 | 18.3/11.6 | 4,878/408 | 1.7/2.8 | 31.0/14.5 | 1,019/575 | 0/0 | 0/0 |
| 8.5 | 1.2/0.9 | 26/30 | 4.2/2.9 | 9.3/11.4 | 268/369 | 0.6/1.9 | 19.4/12.0 | 557/391 | 1.3/2.0 | 31.8/15.7 | 1,143/681 | 0/0.2 | 0/6 |
| 9.5 | 0.9/1.0 | 20/28 | 4.0/3.0 | 10.0/11.7 | 231/338 | 1.8/0.4 | 21.3/12.4 | 493/359 | 1.9/1.2 | 38.7/19.0 | 1,238/681 | 0/1.4 | 0/40 |