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| Gene | Sense | Sequence (5’ → 3’) |
|---|---|---|
| RUNX2-I | Sense | ATGCGTATTCCCGTAGATCC |
| Antisense | GGGCTCACGTCGCTCATTT | |
| RUNX2-II | Sense | AGGAGGGACTATGGCATCAAAC |
| Antisense | GGGCTCACGTCGCTCATTT | |
| RUNX2 (flanking shRNA binding site) | Sense | CACCACTCACTACCACACCT |
| Antisense | AGCATTCTGGAAGGAGACCG | |
| GAPDH | Sense | TCCTCTGACTTCAACAGCGACA |
| Antisense | GTGGTCGTTGAGGGCAATG | |
| TBP | Sense | CACGAACCACGGCACTGATT |
| Antisense | TTTTCTTGCTGCCAGTCTGGAC | |
| ACTB | Sense | ATGACCCAGATCATGTTTGAGA |
| Antisense | AGAGGCGTACAGGGATAGCA |
