| Strain, strain background (Saccharomyces cerevisiae) |
cdc23-1, MIF2-GFP, mRuby2-TUB1 |
This paper |
DDY 5806 |
MATa, cdc23-1, MIF2-GFP::KanMX, TUB1::PHIS3-mRuby2-TUB1::HPH, his3-Δ200, leu2-3,112, ura3-52 |
| Strain, strain background (Saccharomyces cerevisiae) |
cdc23-1, MIF2-GFP, mRuby2-TUB1 |
This paper |
DDY 5807 |
MATalpha, cdc23-1, MIF2-GFP::KanMX, TUB1::PHIS3-mRuby2-TUB1::HPH, his3-Δ200, leu2-3,112, lys2-801(am), ura3-52 |
| Strain, strain background (Saccharomyces cerevisiae) |
cdc23-1, MTW1-GFP, mRuby2-TUB1 |
This paper |
DDY 5808 |
MATa, cdc23-1, MTW1-GFP::KanMX, TUB1::PHIS3-mRuby2-TUB1::HPH, his3-Δ200, leu2-3,112, lys2-801(am), ura3-52 |
| Strain, strain background (Saccharomyces cerevisiae) |
cdc23-1, MTW1-GFP, mRuby2-TUB1 |
This paper |
DDY 5809 |
MATalpha, cdc23-1, MTW1-GFP::KanMX, TUB1::PHIS3-mRuby2-TUB1::HPH, his3-Δ200, leu2-3,112, lys2-801(am), ura3-52 |
| Strain, strain background (Saccharomyces cerevisiae) |
cdc23-1, CSE4-GFP, mRuby2-TUB1 |
This paper |
DDY 5810 |
MATa, cdc23-1, CSE4-3x flexible linker-yoEGFP::KanMX, mRuby2-TUB1::URA3, his3-Δ200, leu2-3,112, lys2-801(am) |
| Strain, strain background (Saccharomyces cerevisiae) |
cdc23-1, CSE4-GFP, mRuby2-TUB1 |
This paper |
DDY 5811 |
MATalpha, cdc23-1, CSE4-3x flexible linker-yoEGFP::KanMX, mRuby2-TUB1::URA3, his3-Δ200, leu2-3,112, lys2-801(am) |
| Strain, strain background (Saccharomyces cerevisiae) |
cdc23-1, OKP1-GFP, mRuby2-TUB1 |
This paper |
DDY 5812 |
MATa, cdc23-1, OKP1-yoEGFP::KanMX, URA3::mRuby2-TUB1, his3-Δ200, leu2-3,112, lys2-801(am) |
| Strain, strain background (Saccharomyces cerevisiae) |
cdc23-1, OKP1-GFP, mRuby2-TUB1 |
This paper |
DDY 5813 |
MATalpha, cdc23-1, OKP1-yoEGFP::KanMX, URA3::mRuby2-TUB1, his3-Δ200, leu2-3,112, lys2-801(am) |
| Strain, strain background (Saccharomyces cerevisiae) |
cdc23-1, CTF3-GFP, mRuby2-TUB1 |
This paper |
DDY 5814 |
MATa, cdc23-1, CTF3-yoEGFP::KanMX, URA3::mRuby2-TUB1, his3-Δ200, leu2-3,112, lys2-801(am) |
| Strain, strain background (Saccharomyces cerevisiae) |
cdc23-1, CTF3-GFP, mRuby2-TUB1 |
This paper |
DDY 5815 |
MATalpha, cdc23-1, CTF3-yoEGFP::KanMX, URA3::mRuby2-TUB1, his3-Δ200, leu2-3,112, lys2-801(am) |
| Strain, strain background (Saccharomyces cerevisiae) |
cdc7-1, SPC105-GFP, mRuby2-TUB1 |
This paper |
DDY 5816 |
MATa, cdc7-1, SPC105-GFP::KanMX, mRuby2-TUB1::HPH, his3-Δ200, leu2-3,112, lys2-801(am), ura3-52 |
| Strain, strain background (Saccharomyces cerevisiae) |
cdc15-2, SPC105-GFP, mRuby2-TUB1 |
This paper |
DDY 5817 |
MATa, cdc15-2, SPC105-GFP::KanMX, mRuby2-TUB1::HPH, his3-Δ200, leu2-3,112, ura3-52 |
| Strain, strain background (Saccharomyces cerevisiae) |
cdc23-1, SPC105-GFP, mRuby2-TUB1 |
This paper |
DDY 5818 |
MATa, cdc23-1, SPC105-3x flexible linker-yoEGFP::KanMX, TUB1::PHIS3-mRuby2-TUB1::HPH, his3-Δ200, leu2-3,112, lys2-801(am), ura3-52 |
| Strain, strain background (Saccharomyces cerevisiae) |
cdc23-1, SPC105-GFP, mRuby2-TUB1 |
This paper |
DDY 5819 |
MATalpha, cdc23-1, SPC105-3x flexible linker-yoEGFP::KanMX, TUB1::PHIS3-mRuby2-TUB1::HPH, his3-Δ200, leu2-3,112, lys2-801(am), ura3-52 |
| Strain, strain background (Saccharomyces cerevisiae) |
cdc23-1, SPC105-GFP, kip3Δ, mRuby2-TUB1 |
This paper |
DDY 5820 |
MATa, cdc23-1, SPC105-3x flexible linker-yoEGFP::KanMX, kip3Δ::HIS3MX6, TUB1::PHIS3-mRuby2-TUB1::HPH, his3-Δ200, leu2-3,112, lys2-801(am), ura3-52 |
| Strain, strain background (Saccharomyces cerevisiae) |
cdc23-1, SPC105-GFP, kip3Δ, mRuby2-TUB1 |
This paper |
DDY 5821 |
MATalpha, cdc23-1, SPC105-3x flexible linker-yoEGFP::KanMX, kip3Δ::HIS3MX6, TUB1::PHIS3-mRuby2-TUB1::HPH, his3-Δ200, leu2-3,112, lys2-801(am), ura3-52 |
| Strain, strain background (Saccharomyces cerevisiae) |
cdc23-1, SPC105-GFP, cin8Δ, mRuby2-TUB1 |
This paper |
DDY 5822 |
MATa, cdc23-1, SPC105-3x flexible linker-yoEGFP::KanMX, cin8Δ::HIS3MX6, TUB1::PHIS3-mRuby2-TUB1::HPH, his3-Δ200, leu2-3,112, lys2-801(am), ura3-52 |
| Strain, strain background (Saccharomyces cerevisiae) |
cdc23-1, SPC105-GFP, cin8Δ, mRuby2-TUB1 |
This paper |
DDY 5823 |
MATalpha, cdc23-1, SPC105-3x flexible linker-yoEGFP::KanMX, cin8Δ::HIS3MX6, TUB1::PHIS3-mRuby2-TUB1::HPH, his3-Δ200, leu2-3,112, lys2-801(am), ura3-52 |
| Strain, strain background (Saccharomyces cerevisiae) |
cdc23-1, NDC80-mScarlet-I, MTW1-GFP, HaloTag-TUB1 |
This paper |
DDY 5824 |
MATa, cdc23-1, NDC80-mScarlet-I::LEU2, MTW1-GFP::KanMX, ura3-52::PHIS3-HaloTag-flexible linker-TUB1::URA3, his3-Δ200, leu2-3,112, lys2-801(am) |
| Strain, strain background (Saccharomyces cerevisiae) |
cdc23-1, NDC80-mScarlet-I, SPC105-GFP, HaloTag-TUB1 |
This paper |
DDY 5825 |
MATa, cdc23-1, NDC80-mScarlet-I::LEU2, SPC105-3x flexible linker-yoEGFP::KanMX, ura3-52::PHIS3-HaloTag-flexible linker-TUB1::URA3, his3-Δ200, leu2-3,112, lys2-801(am) |
| Strain, strain background (Saccharomyces cerevisiae) |
cdc23-1, NDC80-mScarlet-I, ASK1-GFP, HaloTag-TUB1 |
This paper |
DDY 5826 |
MATa, cdc23-1, NDC80-mScarlet-I::LEU2, ASK1-yoEGFP::KanMX, ura3-52::PHIS3-HaloTag-flexible linker-TUB1::URA3, his3-Δ200, leu2-3,112, lys2-801(am) |
| Strain, strain background (Saccharomyces cerevisiae) |
cdc23-1, NDC80-mScarlet-I, MIF2-GFP, HaloTag-TUB1 |
This paper |
DDY 5827 |
MATa, cdc23-1, NDC80-mScarlet-I::LEU2, MIF2-GFP::KanMX, ura3-52::PHIS3-HaloTag-flexible linker-TUB1::URA3, his3-Δ200, leu2-3,112 |
| Strain, strain background (Saccharomyces cerevisiae) |
cdc23-1, MTW1-mScarlet-I, OKP1-GFP, HaloTag-TUB1 |
This paper |
DDY 5828 |
MATalpha, cdc23-1, MTW1-mScarlet-I::LEU2, Okp1-yoEGFP::KanMX, ura3::PHis3-HaloTag-TUB1::URA3, his3-Δ200, leu2-3,112, lys2-801(am) |
| Strain, strain background (Saccharomyces cerevisiae) |
cdc23-1, NDC80-mScarlet-I, CSE4-GFP, HaloTag-TUB1 |
This paper |
DDY 5829 |
MATa, cdc23-1, NDC80-mScarlet-I::LEU2, CSE4-3x flexible linker-yoEGFP::KanMX, ura3-52::PHIS3-HaloTag-flexible linker-TUB1::URA3, his3-Δ200, leu2-3,112, lys2-801(am) |
| Strain, strain background (Saccharomyces cerevisiae) |
cdc23-1, NDC80-mScarlet-I, CTF3-GFP, HaloTag-TUB1 |
This paper |
DDY 5830 |
MATa, cdc23-1, NDC80-mScarlet-I::LEU2, CTF3-yoEGFP::KanMX, ura3-52::PHIS3-HaloTag-flexible linker-TUB1::URA3, his3-Δ200, leu2-3,112, lys2-801(am) |
| Strain, strain background (Saccharomyces cerevisiae) |
cdc23-1, SPC105-mScarlet-I, KIP3-GFP, HaloTag-TUB1 |
This paper |
DDY 5831 |
MATa, cdc23-1, SPC105-mScarlet-I::LEU2, KIP3-yoEGFP::KanMX, ura3-52::PHIS3-HaloTag-TUB1::URA3, his3-Δ200, leu2-3,112, lys2-801(am) |
| Strain, strain background (Saccharomyces cerevisiae) |
cdc23-1, SPC105-mScarlet-I, CIN8-GFP, HaloTag-TUB1 |
This paper |
DDY 5832 |
MATalpha, cdc23-1, SPC105-mScarlet-I::LEU2, CIN8-GFP::KanMX, ura3-52::PHIS3-HaloTag-TUB1::URA3, his3-Δ200, leu2-3,112 |
| Strain, strain background (Saccharomyces cerevisiae) |
cdc23-1, SPC105-mScarlet-I, KIP1-GFP, HaloTag-TUB1 |
This paper |
DDY 5833 |
MATa, cdc23-1, SPC105-mScarlet-I::LEU2, KIP1-GFP::KanMX, ura3-52::PHIS3-HaloTag-TUB1::URA3, his3-Δ200, leu2-3,112, lys2-801(am) |
| Strain, strain background (Saccharomyces cerevisiae) |
cdc23-1, kip3 480 GCN4 coiled-coil-EYFP, SPC105-mScarlet-I, KIP1-GFP, HaloTag-TUB1 |
This paper |
DDY 5835 |
MATalpha, cdc23-1, kip3Δ::KanR::pKIP3-kip3 480 GCN4 coiled-coil-EYFP-tADH::LEU2, SPC105-mScarlet-I::LEU2, his3-Δ200, leu2-3,112, lys2-801(am), ura3-52 |
| Strain, strain background (Saccharomyces cerevisiae) |
cdc23-1, KIP2-GFP, SPC105-mScarlet-I, HaloTag-TUB1 |
This paper |
DDY 5836 |
MATa, cdc23-1, KIP2-3x flexible linker-yoEGFP::KanMX, SPC105-mScarlet-I::LEU2, ura3-52::PHIS3-HaloTag-TUB1::URA3, his3-Δ200, leu2-3,112, lys2-801(am) |
| Strain, strain background (Saccharomyces cerevisiae) |
cdc23-1, kip2Δ, SPC105-GFP, mRuby2-TUB1 |
This paper |
DDY 5837 |
MATa, cdc23-1, kip2Δ::CgHIS3, SPC105-3x flex linker-yoEGFP::KanMX, TUB1::PHIS3-mRuby2-TUB1::HPH, his3-Δ200, leu2-3,112, lys2-801(am), ura3-52 |
| Strain, strain background (Saccharomyces cerevisiae) |
cdc23-1, kip2Δ, SPC105-GFP, mRuby2-TUB1 |
This paper |
DDY 5838 |
MATalpha, cdc23-1, kip2Δ::CgHIS3, SPC105-3x flex linker-yoEGFP::KanMX, TUB1::PHIS3-mRuby2-TUB1::HPH, his3-Δ200, leu2-3,112, lys2-801(am), ura3-52 |
| Strain, strain background (Saccharomyces cerevisiae) |
cdc23-1, kip1Δ, SPC105-GFP, mRuby2-TUB1 |
This paper |
DDY 5840 |
MATalpha, cdc23-1, SPC105-GFP::kanMX, TUB1::PHIS3-mRuby2-TUB1::HPH, KIP1Δ::HIS3M × 6, his3-Δ200, leu2-3,112, lys2-801(am), ura3-52 |
| Strain, strain background (Saccharomyces cerevisiae) |
cdc23-1, kip1Δ, SPC105-GFP, mRuby2-TUB1 |
This paper |
DDY 5841 |
MATa, cdc23-1, SPC105-GFP::kanMX, TUB1::PHIS3-mRuby2-TUB1::HPH, KIP1Δ::HIS3M × 6, his3-Δ200, leu2-3,112, lys2-801(am), ura3-52 |
| Strain, strain background (Saccharomyces cerevisiae) |
cdc23-1, ASK1-GFP, mRuby2-TUB1 |
This paper |
DDY 5842 |
MATalpha, his3Δ200, lys2-801(am) am, ura3-52, leu2-3,112, ASK1-EGFP::kanMX, TUB1::PHIS3-mRuby2-TUB1::HPH, cdc23-1 |
| Strain, strain background (Saccharomyces cerevisiae) |
cdc23-1, ASK1-GFP, mRuby2-TUB1 |
This paper |
DDY 5843 |
MATa, his3Δ200, lys2-801(am), ura3-52, leu2-3,112, ASK1-EGFP::kanMX, TUB1::PHIS3-mRuby2-TUB1::HPH, cdc23-1 |
| Strain, strain background (Saccharomyces cerevisiae) |
cdc23-1, KAR3-AID, TIR1, SPC105-GFP, mRuby2-TUB1 |
This paper |
DDY 5844 |
MATalpha, lys2, KAR3-AID-9myc::HIS3, TIR1::LEU2, SPC105-GFP::kanMX, TUB1::PHIS3-mRuby2-TUB1::HPH, cdc23-1, his3-Δ200, leu2-3,112, lys2-801(am), ura3-52 |
| Strain, strain background (Saccharomyces cerevisiae) |
cdc23-1, KAR3-AID, TIR1, SPC105-GFP, mRuby2-TUB1 |
This paper |
DDY 5845 |
MATalpha, lys2, KAR3-AID-9myc::HIS3, TIR1::LEU2, SPC105-GFP::kanMX, TUB1::PHIS3-mRuby2-TUB1::HPH, cdc23-1, his3-Δ200, leu2-3,112, lys2-801(am), ura3-52 |
| Strain, strain background (Saccharomyces cerevisiae) |
cdc23-1, NDC80-GFP, mRuby2-TUB1 |
This paper |
DDY 5846 |
MATalpha, his3Δ200, lys2-801(am), ura3-52, leu2-3,112, NDC80-EGFP::kanMX, TUB1::PHIS3-mRuby2-TUB1::HPH, cdc23-1 |
| Strain, strain background (Saccharomyces cerevisiae) |
cdc23-1, NDC80-GFP, mRuby2-TUB1 |
This paper |
DDY 5847 |
MATa, his3Δ200, lys2-801(am), ura3-52, leu2-3,112, NDC80-EGFP::kanMX, TUB1::PHIS3-mRuby2-TUB1::HPH, cdc23-1 |
| Strain, strain background (Saccharomyces cerevisiae) |
cdc23-1, CNN1-GFP, mRuby2-TUB1 |
This paper |
DDY 5848 |
MATalpha, his3Δ200, lys2-801(am), ura3-52, leu2-3,112, CNN1-EGFP::kanMX, TUB1::PHIS3-mRuby2-TUB1::HPH, cdc23-1 |
| Strain, strain background (Saccharomyces cerevisiae) |
cdc23-1, CNN1-GFP, mRuby2-TUB1 |
This paper |
DDY 5849 |
MATalpha, his3Δ200, lys2-801(am), ura3-52, leu2-3,112, CNN1-EGFP::kanMX, TUB1::PHIS3-mRuby2-TUB1::HPH, cdc23-1 |
| Strain, strain background (Saccharomyces cerevisiae) |
cdc23-1, GFP-KAR3, SPC105-mScarlet-I |
This paper |
DDY 5850 |
MATa, EGFP-KAR3::KanMX, SPC105-mScarlet-I::LEU2, cdc23-1, his3-Δ200, leu2-3,112, lys2-801(am), ura3-52 |
| Strain, strain background (Saccharomyces cerevisiae) |
cdc23-1, MIF2-GFP, MTW1-mScarlet-I |
This paper |
DDY 5851 |
MATalpha, MIF2-GFP::KanMX, MTW1-mScarlet-I::LEU2, cdc23-1, lys2-801(am), ura3-52, leu2-3,112, his3Δ200 |
| Chemical compound, drug |
Benzonase, purity >90% |
EMD Millipore |
Cat. Num: 70746–3 |
|
| Chemical compound, drug |
Concavalin A |
Sigma-Aldrich |
Cat. Num: C2010 |
|
| Chemical compound, drug |
GMPCPP |
Jena Bioscience |
Cat. Num: NU-405 |
|
| Chemical compound, drug |
Hellmanex III |
Hellma Analytics |
Cat. Num: 9-307-011-4-507 |
|
| Chemical compound, drug |
3-indole acetic acid, 98% |
Sigma-Aldrich |
Cat. Num: I3750 |
|
| Chemical compound, drug |
Janelia Fluor 646 HaloTag ligand |
Promega |
Cat. Num: GA1120 |
|
| Chemical compound, drug |
poly-L-lysine grafted with polyethylene glycol |
SuSoS AG |
PLL(20)-g[3.5]-PEG(2) |
|
| Chemical compound, drug |
poly-L-lysine grafted with polyethylene glycol-biotin (50%) |
SuSoS AG |
PLL(20)-g[3.5]-PEG(2)/PEG(3.4)-biotin(50%) |
|
| Chemical compound, drug |
protease inhibitor cocktail IV |
Calbiochem |
Cat. Num: 539,136 |
|
| Other |
BSA gold tracer, 10 nm |
Electron Microscopy Science |
Cat. Num: 25,486 |
Used for electron microscopy |
| Other |
holey carbon electron microscopy grid, Quantifoil R 2/1, copper grid |
Electron Microscopy Science |
Cat. Num: Q210CR1 |
Used for electron microscopy |
| Recombinant protein |
Tubulin protein (HiLyte Fluor 647) - Porcine brain |
Cytoskeleton |
Cat. Num: TL670M |
|
| Recombinant protein |
Tubulin protein (Biotin) - Porcine brain |
Cytoskeleton |
Cat. Num: T333P |
|
| Software, algorithm |
Fiji/ImageJ |
NIH |
|
https://imagej.net/
|
| Software, algorithm |
IMOD |
University of Colorado |
|
https://bio3d.colorado.edu/imod/
|
| Software, algorithm |
Prism |
Graphpad |
|
|
