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. 2022 Jul 2;17:2893–2905. doi: 10.2147/IJN.S363990

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© 2022 Chen et al.

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Investigation of cellular association and transfection efficiency of mRNA-LNPs in vitro. (A and B) Green fluorescent imaging of HEK293T and DC2.4 cells after transfection of RNA-LNP. The RNA-LNP constituted with DOTAP/DOPE/cholesterol lipoplex and EGFP-RNA at various charge ratios. Fluorescent images were collected after transfection from three time points, 24, 48 and 72 h. (C) The fluorescent images of HEK293T and DC2.4 cells after simultaneous transfection of three RNA-LNP, ie DOTAP-DOPE, DOTAP-Chol and LNP3, respectively. (D) Green fluorescent imaging of HEK293T cells after transfection of RNA-LNP3 modified with different molar quantities of DSPE-PEG₂₀₀₀, and the images were collected at three time points, 24, 48 and 72 h. The scale bar was 100 μm.