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. 2022 Jul 2;17:2893–2905. doi: 10.2147/IJN.S363990

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© 2022 Chen et al.

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Transfection activity investigation of mRNA-LNP3 after lyophilization and rehydration using Opti-mem as formulated buffer. (A) Effect of sucrose concentration on EGFP mRNA delivery and expression to HEK293T cells mediated by LNP3 during lyophilization and rehydration. (B) Effect on EGFP mRNA-LNP3 transfection to HEK293T cells after lyophilization and storage for a week at different temperatures, in this work, sucrose concentration was 10% (w/v). w/v represents mass to volume ratio, RT represent room temperature. The fluorescent images were collected from three time points after transfection, 24, 48 and 72 h. The scale bar was 100 μm.