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. 2001 Jan;67(1):403–410. doi: 10.1128/AEM.67.1.403-410.2001

FIG. 2.

FIG. 2

Detection of reporter gene expression by X-Gal hydrolysis on solid media. (a) Strains EB106 (pSWEET) and EB107 (pSWEET-bgaB) were plated on LB–CHL–X-Gal in the presence of 2% xylose. (b) Strains EB103 (pSPAC-bgaB), EB104 (pSPAC-bgaB ΔlacI), and EB105 (pSPAC) were plated on LB–CHL–X-Gal in the presence of 1 mM IPTG. (c) All five strains were plated on LB–CHL–X-Gal in the absence of an inducer. Strains were grown overnight at 37°C and then incubated at 55°C for color development (up to 36 h).