FIG. 5.

Xylose-dependent complementation of a temperature-sensitive mutant in teichoic acid biosynthesis. Strains were plated on LB agar in the presence or absence of an inducer and incubated at 30°C (permissive temperature) or 47°C (nonpermissive temperature). Strains EB4 (tag-12), EB6 (tag⁺), EB123 (tag-12 pSWEET-tagD), and EB127 (tag-12 pSPAC-tagD) were plated at 30°C (a), plated at 47°C (b), supplemented with 1 mM IPTG at 47°C (c), and supplemented with 2% xylose at 47°C (d). Strains shown in panels b through d were plated as indicated in panel a.