TABLE 1.

List of strains and plasmids

Strain or plasmid	Description	Reference or source
B. subtilis
EB161	trpC2 pheA1 amyE::ery	Bacillus Genetic Stock Center 1A717
EB106	trpC2 pheA1 amyE::xylR PxylA cat86	This work
EB107	trpC2 pheA1 amyE::xylR PxylA bgaB cat86	This work
EB105	trpC2 pheA1 amyE::Pspac lacI cat86	This work
EB104	trpC2 pheA1 amyE::Pspac bgaB cat86	This work
EB103	trpC2 pheA1 amyE::Pspac bgaB lacI cat86	This work
EB6	hisA1 argC4 metC3	L5087 (2)
EB4a	hisA1 argC4 metC3 tag-12 φ29r	L6602 (2)
EB123	hisA1 argC4 metC3 tag-12 φ29ramyE::xylR PxylA tagD cat86	This work
EB127	hisA1 argC4 metC3 tag-12 φ29ramyE::Pspac tagD lacI cat86	This work
E. coli
Novablueb	endA1 hsdR17 (rK12− mK12+) supE44 thi-1 recA1 gyrA96 relA1 lac[F′ proA+B+ lacIqZΔM15::Tn10(TcR)]	Novagen
Plasmids
pDG364	B. subtilis vector for integration at amyE	Bacillus Genetic Stock Center (3)
pSWEET	pDG364 with xyl expression system	This work
pSWEET-bgaB	pSWEET with bgaB from B. stearothermophilus and polylinker	This work
pSWEET-tagD	pSWEET with wild-type tagD from B. subtilis 168	This work
pDR67	Plasmid containing spac expression system for integration at amyE	15
pSPAC-bgaB ΔlacI	pDR67 with bgaB from B. stearothermophilus without lacI gene	This work
pSPAC-bgaB	pDR67 with bgaB from B. stearothermophilus	This work
pSPAC-tagD	pDR67 with tagD from B. subtilis 168	This work

^aTemperature-sensitive mutant in teichoic acid biosynthesis ascribed to tagD. 

^bCloning strain.