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Quantitative primer information.
| Gene | Primer sequences (5′⟶3′) | Product length (bp) |
|---|---|---|
| PTCH1 | F: GAAGAAGGTGCTAATGTCCTGAC R: GTCCCAGACTGTAATTTCGCC |
245 |
|
| ||
| GLI1 | F: AGCGTGAGCCTGAATCTGTG R: CAGCATGTACTGGGCTTTGAA |
188 |
|
| ||
| GLI2 | F: CATGGAGCACTACCTCCGTTC R: CGAGGGTCATCTGGTGGTAAT |
173 |
|
| ||
| Hes1 | F: TCAACACGACACCGGATAAAC R: GCCGCGAGCTATCTTTCTTCA |
153 |
|
| ||
| SHH | F: GAAACTCCGAGCGATTTAAGGA R: GGCCCTCGTAGTGCAGAGA |
228 |
|
| ||
| β-Actin | F: GGGATTTCGACTTTTGGCTACA R: CTTCCACTACTGCTGTTAGGTG |
113 |
