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. 2022 Mar 28;15(4):668–682. doi: 10.1038/s41385-022-00503-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Flow cytometric analyses of the frequencies of KLF2:GFP-positive cells within gated TACI⁺/CD138⁺ PB/PC in BM, spleen and mLN (left panel), within gated TACI⁺/CD138⁺/IgA⁺, TACI⁺/CD138⁺/IgM⁺ and TACI⁺/CD138⁺/IgA⁻/IgM⁻ (DN) PB/PC (upper right panel) as well as in PB/PC fractions P1 (TACI⁺/CD138⁺/CD19⁺/B220⁺), P2 (TACI⁺/CD138⁺/CD19⁺/B220^neg) and P3 (TACI⁺/CD138⁺/CD19^int/neg/B220^neg) (lower right panel). Numbers indicate the percentages of cells in the respective gates. b Bar charts to the left represent the arithmetic mean values ± SEM of frequencies of KLF2:GFP-expressing cells within IgA⁺, IgM⁺ and DN PB/PC subsets in BM, spleen (SP) and mLN from n = 5 mice. Bar charts to the right represent the arithmetic mean values mice with SEM of frequencies of KLF2:GFP-expressing cells within IgA⁺ P1, P2 and P3 PB/PC subsets in BM, spleen and mLN from n = 5 mice. c TaqMan-PCR analyses of KLF2 transcripts of sorter-purified PC derived from BM, SP and mLN of C57BL/6 mice. Bar charts represent the arithmetic mean values of KLF2 RNA abundances in TACI⁺/CD138⁺ IgA⁺ or IgM⁺ or DN sorted PB/PC from the BM, SP or mLN of C57BL/6 mice normalized to GAPDH expression. Statistical analyses in b and c were performed for PC subset or isotype comparison by two-way ANOVA with Sidak’s correction for multiple comparisons. FI Fluorescence intensity, FS Forward Scatter, SS Sideward Scatter, BM bone marrow, SP spleen, mLN mesenteric lymph nodes, FO B follicular B cells, MZ B Marginal Zone B cells, ns non-significant; *p < 0.05; ***p < 0.005; ****p < 0.001.