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. 2022 Jun 22;607(7917):191–196. doi: 10.1038/s41586-022-04859-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 2 — a, Workflow used to generate the OMCC C1 Ab Initio and 3.28 Å map. These maps were used for symmetry analysis of the OMCC O-layer and I-layer. b, workflow used to generate the O-layer C14 2.48 Å map and the I-layer C16 3.08 Å map. These maps were used to build and refine the atomic O- and I-layer models, respectively. c, workflow used to generate the initial Ab Initio model for the IMC-Arches-Stalk map. d, workflow used to generate the IMC-Arches-Stalk C1 6.18 Å map which was used 1- to build the Arches model, 2- for symmetry analysis of the IMC, the Arches, and the Stalk, 3- to derive the IMC protomer C1 3.75 Å map (used to build the IMC protomer model), 4- to derive the Stalk C1 3.71 Å and the Stalk C5 3.28 Å maps (used to build the Stalk model).