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. 2022 Jul 6;13:3709. doi: 10.1038/s41467-022-31299-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — smFRET measurements (a, d) were used to build histograms of the donor lifetime, which shortens as the donor-acceptor distance decreases. Dotted lines indicate the medians with corresponding distances on upper x-axis. a The donor fluorescence lifetime distribution in DMPC (PC) bilayer without ligand (top); with 1 μM EGF (second); with 1 μM EGF and 100 nM Cetuximab (third); with 1 μM epigen (bottom). b Molecular dynamics trajectories were used to find the probability density distribution of the vertical separation in a PC bilayer (top) or a POPC-POPS (PC/PS) bilayer (bottom) without EGF (purple) and with EGF (orange). Dotted lines indicate the median of the distributions (PC bilayer: 9.2 nm (-EGF) and 7.5 nm (+EGF); PC/PS bilayer: 7.4 nm (−EGF) and 9.0 nm (+EGF)). The shaded regions represent the standard deviation estimated with block averaging. c Zeta potential distributions for EGFR in PC (dark purple) and PC/PS (light purple) nanodiscs. d The donor fluorescence lifetime distribution in PC/PS bilayer without ligand (top); with 1 μM EGF (second); with 1 μM EGF and 100 nM Cetuximab (third); with 1 μM epigen (bottom). Source data are provided as a Source data file.