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. 2022 Jul 6;13:3884. doi: 10.1038/s41467-022-31584-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Schematic diagram of the D₂O-labeled single-cell Raman technique. 1 represents the incubation and preparation of microorganism liquids; 2 represents Raman spectroscopy. Raman characteristic bands assigned to intracellular lipid/fatty acid biomolecules are pinpointed. b The ratios of (C–D)/(C–D + C–H) calculated from 40 to 50 single cells in the plastisphere and bulk water. Each point is a single cell. Different letters (a and b) indicate the significant differences (P < 0.05) among each plastisphere group and bulk water group.