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. 2022 Jul 6;13:3884. doi: 10.1038/s41467-022-31584-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Concentrations of N₂O after the 24 h denitrification experiment. b Denitrification rates quantified by ¹⁵N isotope-pairing technique. Both N₂O production and denitrification rate are normalized with microbial cell numbers by using the absolute abundances of 16S rRNA in the plastisphere and bulk water (see “Methods”). c Contributions of denitrification and Anammox processes to N₂ production. Error bars are the standard error. Different letters (a, b, and c) indicate the significant differences (P < 0.05) among each plastisphere group and bulk water group.