Skip to main content
. 2022 Jul 6;13(7):583. doi: 10.1038/s41419-022-05047-6

Fig. 3. Pol β regulates pyroptosis in macrophages.

Fig. 3

A, B RAW264.7 cells with stable knockdown or overexpression of Pol β or BMDMs from Pol β+/− mice were treated with 1 μg/mL LPS for 4 h, and 5 mM ATP was then added and incubated for an additional 1 h, and the levels of NLRP3, GSDMD-N, and caspase-1 expression were determined by Western blotting. C, D Representative images of Hoechst 33342 and PI double fluorescent staining. Scale bar, 50 μm. E, F LDH release levels in RAW264.7 cells after pyroptosis induction. Data present mean ± SEM of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, one-way ANOVA. ELISA analysis of IL-1β and IL-18 secreted by RAW264.7 cells with stable knockdown or overexpression of Pol β (G, H) or BMDMs from Pol β+/− mice (I, J) after LPS plus ATP treatment. Data present mean ± SEM of three independent experiments *P < 0.05, **P < 0.01, ***P < 0.001, one-way ANOVA.