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. 2022 Jul 6;13(7):583. doi: 10.1038/s41419-022-05047-6

Fig. 4. Pol β suppresses knockdown exacerbates DNA damage and decreases the leakage of dsDNA into the cytoplasm in pyroptotic macrophages.

Fig. 4

A Representative alkaline comet images of DNA damage in RAW264.7 cells after LPS and ATP treatment. B Percentage of DNA in the tails was quantified in panel A. Data represent mean ± SEM of three independent experiments. **P < 0.01, ***P < 0.001, one-way ANOVA. C Representative Western blot of γ-H2AX in RAW264.7 cells with stable knockdown or overexpression of Pol β after LPS plus ATP treatment. D Representative alkaline comet images of DNA damage in BMDM cells from WT and Pol β+/− mice after LPS and ATP treatment. E Percentage of DNA in the tails was quantified in panel D. Data represent mean ± SEM of three independent experiments. ***P < 0.001, one-way ANOVA. F Representative Western blot of γ-H2AX in BMDM cells after LPS plus ATP treatment. Representative immunofluorescence images of γ-H2AX and cytosolic dsDNA foci in RAW264.7 cells (G, H) and BMDM cells (I, J) after treatment with LPS plus ATP. Lower panel: statistical analysis, data represent the mean ± SEM of three independent experiments; *P < 0.05, ***P < 0.001, one-way ANOVA. Scale bar, 10 μm.