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. 2022 Jul 6;13:3901. doi: 10.1038/s41467-022-31614-8

Fig. 5. Advanced regulation of endogenous carbon utilization genes.

Fig. 5

a Wiring diagram for an AND gate controlling a sgRNA output targeted to an endogenous gene (left). The sgRNA is the same as in Fig. 4e. Growth curves are shown for B. uniformis harboring this circuit when grown in inulin minimal media in the presence of all combinations of both inducers (10 mM IPTG and 1 mM D-ribose) (right). b Similar to a, but for a NOR gate controlling a sgRNA targeted to the B. ovatus SusC-like gene involved in inulin import. c Similar to b, but for an OR gate controlling the sgRNA output. Source data are provided as a Source Data file. For OD600 growth curves, data represent the average of n = 3 biological replicates. Error bars correspond to the SEM of these measurements.