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. 2022 Jun 23;10:846254. doi: 10.3389/fped.2022.846254

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Copyright © 2022 Talavera-Barber, Flint, Graber, Dhital, Kaptsan, Medoro, Sánchez and Shimamura.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Neutralizing titers of maternal and infant sera. CMV neutralizing capacity of maternal third trimester sera and infant cord sera were tested using an in-vitro neutralizing assay, without or with guinea pig complement, and the IC50 titer was calculated for each sample. The IC50 titers without complement (dotted lines) and with complement (solid lines) were compared to anti-gB and anti-gM/gN titers in maternal (A,C) and infant (B,D) sera. (A,B) The x-axis range reflects anti-gB titers ranging from 1:200-1:12800. (C,D) The x-axis range reflects anti-gM/gN titers ranging from 0–1:800 (A–D). For each correlation plot, a single circle/square may represent more than one sample with the antibody titer/neutralizing IC50 titer shown in each graph.