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. 2022 Jul 4;5(11):e202201366. doi: 10.26508/lsa.202201366

Figure 5. HSPG gene knockdown rescues motor function and reduces α-synuclein aggregation in pre-formed fibril (PFF)-fed animals.

Figure 5.

(A) Worms expressing human wild-type α-synuclein–YFP in muscle cells (strain NL5901) were fed 0.25 ng/μl PFFs or buffer on day 1 of adulthood and motor assays were conducted on day 4. n = 38 for hst-1 and 19 for each of the other groups. One-way ANOVA with Dunnett’s post hoc test. (B), On day 4, α-synuclein aggregates (arrows) were imaged. Scale bar, 50 μm. (C) Quantification of # α-synuclein aggregates per worm normalized to the corresponding Vect group: values for PFF-fed groups are expressed as % of PFF-fed Vect control group, and values for Buffer-treated groups are expressed as % of Buffer-treated Vect control group. n = 9 each for sdn-1 Buffer and hst-1 groups, n = 8 each for hst-3.2 Buffer and rib-2 groups, n = 7 for rib-1 Buffer, n = 10 each for sdn-1 PFF and hst-3.2 PFF, n = 13 for rib-1 PFF. Two-tailed t test. Vect, empty vector RNAi. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Boxplots show minimum, 25th percentile, median, 75th percentile, and maximum.