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. 2022 Jul 4;5(11):e202201366. doi: 10.26508/lsa.202201366

Figure 6. Pre-formed fibril (PFF)-induced dopaminergic neurodegeneration is mitigated by HSPG gene knockdown.

Figure 6.

(A) Worms expressing pan-neuronal human wild-type α-synuclein in a neuronal RNAi-sensitive background (strain MOR002) were treated with 0.5 ng/μl PFFs on day 1 of adulthood. On day 2, dopamine neurons were imaged and scored for rescue of cell bodies. Scale bar, 20 μm. (B) Quantification of CEP cell body area normalized to the corresponding Vect group: values for PFF-fed groups are expressed as % of PFF-fed Vect control group, and values for Buffer-treated groups are expressed as % of Buffer-treated Vect control group. n = 28 for each group. Two-tailed t test. (C) Quantification of number of CEP cell bodies. Data are mean ± SEM. n = 7 for each group. One-way ANOVA with Dunnett’s post hoc test. (D) Quantification of ADE cell body area normalized to the corresponding Vect group: values for PFF-fed groups are expressed as % of PFF-fed Vect control group, and values for Buffer-treated groups are expressed as % of Buffer-treated Vect control group. n = 14 for each group. Two-tailed t test. (E) Quantification of number of ADE cell bodies. Data are mean ± SEM. n = 7 for each group. One-way ANOVA with Dunnett’s post hoc test. Vect, empty vector RNAi. *P < 0.05, **P < 0.01, ***P < 0.001. Boxplots show minimum, 25th percentile, median, 75th percentile, and maximum.