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. 2022 Jul 4;5(11):e202201366. doi: 10.26508/lsa.202201366

Figure S5. RNAi controls for motor function screening.

Figure S5.

(A) Real-time PCR data for hse-5 and hst-2 mRNA expression shows successful knockdown in both cases. For hse-5 measurement, n = 3 for Vect and 5 for hse-5. For hst-2 measurement, n = 4 for Vect and 6 for hst-2. Data are normalized to Vect. Two-tailed t test. (B) Knockdown of HSPG genes in buffer-treated NL5901 worms shows no effect on motor function on day 4. n = 19 each for hst-1 and sdn-1, n = 18 for Vect, 14 for rib-1, 13 for rib-2, and 16 for hst-3.2. One-way ANOVA with Dunnett’s post hoc test. ns, not significant. Vect, empty vector RNAi. *P < 0.05, ***P < 0.001. Boxplots show minimum, 25th percentile, median, 75th percentile, and maximum.