TABLE 1.
Primers used for semiquantitative real-time PCR.
| Gene | Forward primer (5′-3′) | Reverse primer (5′-3′) | Annealing temp (°C) | Amplicon size (bp) |
| Actb | CAACGGCTCCGGCATGTGC | CTCTTGCTCTGGGCCTCG | 62 | 153 |
| Adm | TTCGCAGTTCCGAAAGAAGT | GGTAGCTGCTGGATGCTTGTA | 62 | 77 |
| Epo | GCCCTGCTAGCCAATTCC | GGCGACATCAATTCCTTCTG | 60 | 128 |
| Gnat1 | GAGGATGCTGAGAAGGATGC | TGAATGTTGAGCGTGGTCAT | 58 | 209 |
| Mct3 | GGCTCAACCCTAAATCCAGA | CTTCGGAGTTTCCTCACCAG | 62 | 75 |
| Opn4 | CCAGCTTCACAACCAGTCCT | CAGCCTGATGTGCAGATGTC | 62 | 111 |
| Stc1 | CCGGAAGCCATCACTGAA | GGCTTCGGACAAGTCTGTTG | 62 | 76 |
| Stc2 | AGCAGGAAGTGTCCAGCAAT | GGTTCACAAGGTCCACATAGG | 62 | 166 |
| Vsx2 | CCAGAAGACAGGATACAGGTG | GGCTCCATAGAGACCATACT | 62 | 111 |