Cloning and sequencing strategy for the genes encoding catabolism of alkylbenzene in P. putida 01G3 (3,013-bp fragment). The directions of translation are indicated by arrowheads. pA32 and pR2B are pBluescriptII SK+ derivatives used for sequencing. Abbreviations: CI, ClaI; BI, BamHI; H3, HindIII; KI, KpnI; SII, SacII; kpb, kilobase pair.