FIGURE 1.

Schematic representation of the screening pipeline proposed. First, optimal buffer and detergent conditions for the stability of a purified membrane protein sample are identified by nDSF. Selected conditions are used for further screening by Dynamic Light Scattering (DLS) to determine the aggregation status of the protein under the specific buffer and detergent conditions. Mass photometry measurements are used to confirm the expected molecular weight of the protein and the distribution of masses present in the sample. Finally, the sample is imaged successively by negative stain and cryo-EM.