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. 2022 Jun 23;9:882288. doi: 10.3389/fmolb.2022.882288

FIGURE 5.

FIGURE 5

Mass photometry of IJ1. For each experiment, 0.5 ul of pre-dilution was added to 19.5 detergent-free buffer on the mass photometry slide. (A) Measurement using a protein pre-dilution with 0.036% DDM. The final DDM concentration is 0.0009% and 40 nM protein. (B) Pre-dilution of protein solution into detergent-free buffer results in a DDM concentration of 0.006%. The final DDM concentration is 0.00015% and 80 nM protein. (C,D) Control experiments using similar DDM concentrations and no protein. (C) 0.03% DDM. (D) 0.003% DDM. (E) and (F) show the mass histograms for IJ1 in the presence of different concentrations of LMNG as detergent at a final protein concentration of 40 nM. Since the protein was concentrated, we can only give estimations for the final LMNG concentrations: 0.00004% for panel E and 0.000015% for panels (F), (G), and (H). Amphipol solubilized IJ1 at two different pre-dilution concentrations: (G) 3.2 uM IJ1 and 0.0042% A8-35. (H) 1.6 uM IJ1 and 0.0021% A8-35. The final protein concentrations were 80 nM (G) and 40 nM (H).