Table 3.
Comparison of the ICSI outcomes between frozen and fresh sperm.
| Frozen sperm (n=257) | Fresh sperm (n=42) | P-value | |
|---|---|---|---|
| Age (yr) | |||
| Male | 31.58 ± 4.91 | 31.10 ± 3.86 | 0.540 |
| Female | 29.01 ± 4.07 | 29.64 ± 3.94 | 0.350 |
| COS-ICSI outcomes | / | / | / |
| Days of ovarian stimulation | 10.62 ± 2.29 | 10.50 ± 1.55 | 0.738 |
| Total gonadotropin dose (IU) | 1914.55 ± 959.68 | 1732.14 ± 617.26 | 0.235 |
| Estradiol level on HCG trigger day (pmol/L) | 10789.34 ± 4528.86 | 13282.10 ± 4550.46 | 0.001* |
| Progesterone level on HCG trigger day (nmol/L) | 2.35 ± 1.30 | 2.77 ± 1.50 | 0.059 |
| The endometrial thickness on HCG trigger day (mm) | 10.56 ± 2.08 | 10.29 ± 1.66 | 0.417 |
| Number of oocytes retrieved | 13.87 ± 7.13 | 17.50 ± 7.23 | 0.002* |
| Number of MII oocytes for ICSI | 11.02 ± 8.29 | 10.62 ± 5.73 | 0.766 |
| Fertilization rate (%) | 70.1 (1985/2831) | 68.2 (304/446) | 0.403 |
| 0.516# | |||
| 2PN rate (%) | 61.8 (1750/2831) | 59.4 (265/446) | 0.333 |
| 0.684# | |||
| 2PN cleavage rate (%) | 91.9 (1609/1750) | 95.1 (252/265) | 0.072 |
| 0.447# | |||
| Number of available embryos on day 3 | 3.09 ± 2.94 | 2.86 ± 2.25 | 0.625 |
| 0.074# | |||
| Number of high-score embryos on day 3 | 0.84 ± 1.15 | 0.98 ± 1.05 | 0.474 |
| 0.899# | |||
| Day 3 utilization rate of MII eggs for ICSI (%)1 | 28.7 (812/2831) | 26.2 (117/446) | 0.286 |
| 0.899# | |||
| Rate of high-score embryo on day 3 (%)2 | 12.3 (216/1750) | 15.5 (41/265) | 0.16 |
| 0.062# | |||
| No. of cycles without available embryos/ICSI cycles (%) | 11.3 (29/257) | 14.3 (6/42) | 0.575 |
| 1st embryo transfer cycle | |||
| No. of embryos transferred | 1.56 ± 0.51 | 1.61 ± 0.55 | 0.533 |
| Clinical pregnancy rate (%) | 45.9 (118/257) | 42.9 (18/42) | 0.712 |
| 0.452# | |||
| Live birth rate (%) | 44.0 (113/257) | 40.5 (17/42) | 0.672 |
| 0.499# | |||
| One ICSI cycle | |||
| Cumulative pregnancy rate per women (%) | 51.0 (131/257) | 45.2 (19/42) | 0.491 |
| Cumulative live birth rate per women (%) | 47.5 (122/257) | 42.9 (18/42) | 0.579 |
| No. live birth/clinical pregnancy (%) | 93.1 (122/131) | 94.7 (18/19) | 0.793 |
| Singleton (%) | 86.9 (106/122) | 88.9 (16/18) | 0.813 |
| Twins (%) | 13.1 (16/122) | 11.1 (2/18) | |
| Birth defect (%) | 1.6 (2/122) | 0 (0/18) | 0.584 |
| Gender | / | / | 0.941 |
| Male (%) | 39.1 (54/138) | 40.0 (8/20) | / |
| Female (%) | 60.9 (84/138) | 60.0 (12/20) | / |
| Birth weight (g) | |||
| Singleton | 3194.67 ± 405.16 | 3038.44 ± 495.32 | 0.166 |
| Twins | 2302.50 ± 331.11 | 2527.50 ± 197.55 | 0.196 |
| Height (cm) | |||
| Singleton | 49.84 ± 2.04 | 48.50 ± 3.03 | 0.024* |
| Twins | 44.91 ± 3.40 | 47.50 ± 1.00 | 0.143 |
Data are expressed as mean ± SD unless indicated otherwise
1 Computational formula: number of available embryos on day3 / number of MII oocytes for ICSI.
2 Computational formula: number of high-score embryos on day 3 / number of 2PN.
*Significantly different.
# P value after correcting the estradiol level on HCG trigger day.
ICSI, intracytoplasmic sperm injection; COS, controlled ovarian stimulation.
P value after corrected the estradiol level on HCG trigger day.