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. 2022 Jun 23;10:873278. doi: 10.3389/fcell.2022.873278

FIGURE 4.

FIGURE 4

Mechano-activation of GPR126 via its ligands. (A) Domain architecture of human GPR126 with the binding sites of its ligands, collagen IV and laminin 211, is depicted. (B,E) Schematic demonstrating the AFM setup with (B) collagen IV- and (E) laminin 211-coated cantilevers. (C,D) Changes in Pink Flamindo fluorescence intensity after applying a varying pushing (C) or pulling (D) force over 60s with a collagen IV–coated cantilever. (F,G) Changes in Pink Flamindo fluorescence intensity after applying a pushing (F) or pulling (G) force over 60s with a laminin 211-coated cantilever. Data are given as the means ± SEM of three different experiments, each measuring three individual cells for each force. Statistics were performed as two-way ANOVA (C): effect of construct p = 0.1452, effect of force applied p = 0.0635, interaction construct × force applied p = 0.0298; (D): effect of construct p = 0.8096, effect of force applied p = 0.1748, interaction construct × force applied p = 0.2744; (F): effect of construct p = 0.0537, effect of force applied p = 0.4536, interaction construct × force applied p = 0,7286; (G): effect of construct p = 0.2225, effect of force applied p = 0.5884, interaction construct × force applied p = 0.0246) in combination with Tukey’s post hoc analysis; *p < 0.05; **p < 0.01. Significances in the graphs show the results of the post hoc analysis. Corresponding raw data can be found in the repository. Schematic images were created with BioRender.com.