Table 2. Comparison of gene expression and percentages of positive cells for tenogenic markers among treatments.
| Group | Comparative gene expression (Mean ± SE) | Percentage of positive cells | |||
|---|---|---|---|---|---|
| TNMD | SCX | DCN | TNC | COL1 | |
| Control | 1.00 ± 0.00a | 1.00 ± 0.00a | 1.00 ± 0.00a | 1.00 ± 0.00a | 0.00% (0/153)a |
| BMP | 11.58 ± 4.34b | 1.36 ± 0.21a | 1.53 ± 0.12a | 1.43 ± 0.17a | 50.32% (78/155)b |
| BMPKY1 | 52.95 ± 19.38c | 2.16 ± 0.36b | 1.60 ± 0.12a | 1.58 ± 0.15a | 53.72% (65/121)bc |
| BMPKY3 | 1.81 ± 0.63a | 0.98 ± 0.05a | 1.36 ± 0.31a | 1.26 ± 0.19a | 65.65% (86/131)c |
| BMPKY5 | 1.55 ± 0.04a | 0.86 ± 0.07a | 3.32 ± 0.51b | 3.27 ± 0.37b | 55.13% (86/156)bc |
Average gene expression was calculated for the three cell lines and repeated in triplicate. a–cSignificant difference (P<0.05). TNMD: Tenomodulin; SCX: Scleraxis; DCN: Decorin; TNC: Tenascin-C; COL1: type I collagen.