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. 2022 Jun 23;9:873163. doi: 10.3389/fvets.2022.873163

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Copyright © 2022 Mescolini, Baigent, Catelli and Nair.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Limit of detection of HVT LAMP-LFD assay. The limit of detection (LoD) was tested using 10-fold serial dilutions of the pHVT-BAC 3 plasmid containing a known number of copies of the HVT genome (10⁰-10⁶). This was repeated in triplicate (replicates not shown). A band at the test line (T) shows a positive result. A band at the control line (C) shows a negative result. The LoD was 10² copies of the HVT genome.