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. 2022 Jun 23;9:873163. doi: 10.3389/fvets.2022.873163

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Copyright © 2022 Mescolini, Baigent, Catelli and Nair.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Vaxxitek®-specific LAMP-LFD assay detects Vaxxitek® but not conventional HVT vaccine strain FC126 or rHVT vaccine Innovax®. The Vaxxitek®-specific LAMP assay was performed in a heating block and using FAM/Biosg-labeled primers and target DNA was prepared from non-infected CEF cells (negative control), or CEF cells infected with HVT strain FC126, the Vaxxitek® recombinant HVT vaccine virus, or the Innovax® recombinant HVT vaccine virus. The reaction mixture was loaded onto a LFD for readout of results. A band at the test line (T) shows a positive result. A band at the control line (C) shows a negative result. Only Vaxxitek® gave a positive result.